Faculty of Public Health - Andalas University - OCS, 13th IEA SEA Meeting and ICPH - SDev

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Accuracy Rapid Test Diagnostics On Dengue Suspec In Hospital Semarang City Indonesia
Martini Martini, Soraya Hidayati, Retno Hestiningsih, Sri Yuliawati, Atik Mawarni, indranilla kustarini

Last modified: 2018-08-07

Abstract


Background

The diagnosis of dengue virus infection is done by anamnesis and physical examination in patient, then followed by inspection of laboratory in the form of checking of thrombocyte and hematocrit level. RDT NS1 and RDT IgG / IgM are used to strengthen the diagnosis of dengue virus infection quickly and easily. This study aims to determine the accuracy of Rapid Diagnostic Test (RDT) for the detection of dengue virus infection in dengue suspect patients in Semarang City

Materials and methods

This study used diagnostic test method in 54 dengue suspect patients using cross sectional study design in 2 hospitals and 7 puskesmas in Semarang City. Data was analyzed using descriptive statistic to know accuracy the test.

 

Results

The results showed patients with thrombocytopenia (79.6%), and hematocrit levels above normal (31.5%). Results of RDT IgG / IgM examination were compared with RT-PCR to obtain sensitivity (66.6%), specificity (77.3%), positive predictive value (28.6%), negative predictive value (94.4%), and accuracy (76%). While RDT NS1 has sensitivity (5%), specificity (97.6%), positive predictive value (50%), negative predictive value (68.3%), and accuracy (67%). Dengue virus strains found were DEN-1 and DEN-3.

 

AKNOWLEDGEMENT

We thank to Higher Educational Ministry of Indonesia for funding the research, and ITD Laboratory helping in detection virus of Dengue using PCR.

Conclusions

The conclusions of this study are that RDT NS1 and RDT IgG / IgM are not accurate for the diagnosis of dengue virus infection.

 

Keywords :  Dengue, RDT NS1, RDT IgG/IgM, RT-PCR